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BrdU Cell Proliferation Chemiluminescent 分析試劑:BrdU Cell Proliferation Chemiluminescent Assa 1Kit

價格:¥

貨号:5492S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

BrdU細胞增殖化學發光檢測試劑盒使用Anti-BrdU抗體檢測細胞增殖時摻入DNA中的5-bromo-2’-deoxyuridine (BrdU)。使用含有BrdU的培養基培養細胞,這種嘧啶類似物能夠在新DNA和成時取代胸腺嘧啶。移除培養基後,使用我們的固定/變性液以固定細胞,變性DNA。DNA變性能提高BrdU的摻入以便被抗體檢測到。然後加入BrdU鼠單抗以檢測摻入的BrdU。Anti-mouse IgG,HRP-偶聯的抗體用以結合抗體。然後加入化學發光試劑。發射光的強度以相對光單位測得(RLU),與摻入細胞的BrdU量成正比,是細胞增殖的直接指示。BrdU細胞增殖化學發光檢測試劑盒能夠檢測在細胞增殖時摻入細胞DNA的BrdU。BrdU标記的DNA必須經過變性才能使用試劑盒中的BrdU Mouse mAb檢測。BrdU Mouse mAb與内源性DNA沒有交叉反應。0.2-2x104 cells/well足以應對大多數實驗類型,細胞數目取決于細胞類型和孵育時間。為了得到最好的結果,建議對細胞進行梯度稀釋
500 assays (96 well format)。 The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody. A BrdU mouse mAb is then added to detect the incorporated BrdU. Anti-mouse IgG, HRP-linked Antibody is used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Chemiluminescent Assay Kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA must be denatured to be detected by the BrdU mouse mAb used in this kit. This BrdU mouse mAb does not cross-react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups. For best results, a cell number titration (Figure 1) is recommended.

BrdU 細胞增殖分析試劑盒:BrdU Cell Proliferation Assay Kit 1Kit

價格:¥

貨号:6813S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

BrdU Cell Proliferation Assay kit能夠檢測細胞增殖過程中摻入到細胞DNA中的BrdU,使用的抗體為抗BrDU抗體。當細胞培養于含BrDU的标記培養基中,該胸腺嘧啶類似物可代替胸腺嘧啶摻入到增殖細胞的新合成DNA中。吸除标記培養基後,用固定/變性液固定細胞同時使DNA變性。然後加入BrdU鼠源單抗檢測摻入的BrdU(DNA變性使得摻入的BrdU易于為抗體所識别)。Anti-mouse IgG, HRP-linked antibody用來識别聯胞檢測抗體。加入HRP底物(TMB)進行顯色。顯色過程中吸光度的強度和摻入細胞的BrdU的量成正比, BrdU是細胞增殖的直接證明。BrdU Cell Proliferation Assay kit能夠檢測細胞增殖過程中摻入到細胞DNA中的BrdU。BrDU标記的DNA變性後能夠為試劑盒中的BrdU Mouse mAb檢測到。BrdU Mouse mAb不與内源性DNA發生交叉反應。依細胞種類和孵育時間不同 ,每孔0.2-2x104個細胞即足夠大部分實驗。為了得到最佳實驗結果,我們推薦進行細胞數滴度實驗
500 assays (96 well format). The BrdU Cell Proliferation Assay Kit detects 5-bromo-2’-deoxyuridine (BrdU) incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are cultured with labeling medium that contains BrdU, this pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. After removing labeling medium, cells are fixed and the DNA is denatured with our fixing/denaturing solution. Then a BrdU mouse mAb is added to detect the incorporated BrdU (The denaturing of DNA is necessary to improve the accessibility of the incorporated BrdU to the detection antibody). Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells, which is a direct indication of cell proliferation.
BrdU Cell Proliferation Assay kit detects BrdU incorporation into cellular DNA during cell proliferation. The BrdU-labeled DNA has to be denatured to be detected by the BrdU Mouse mAb used in this kit. This BrdU Mouse mAb does not cross react with endogenous DNA. Depending on the cell type and the incubation time applied in the assay, 0.2-2x104 cells/well are sufficient for most experimental setups.

CCK-8 Cell Counting Kit 500 rxn

價格:¥

貨号:A311-01
品牌:Vazyme

經銷:南京諾唯贊生物

CCK-8 Cell Counting Kit, 簡稱CCK-8 試劑盒, 是依賴于WST-8 (2-(2- 甲氧基-4- 硝苯基)-3-(4- 硝苯基)-5-(2,4- 二磺基苯)-2H- 四唑單鈉鹽) 而廣泛應用于細胞增殖和細胞毒性檢測的快速、高靈敏度、無放射性的比色檢測試劑盒。WST-8 在電子耦合載體1-Methoxy PMS 的作用下可被線粒體内的一些脫氫酶還原成高度水溶性的橙黃色甲臜産物(Formazan),生成的甲臜的顔色深淺與細胞增殖成正比,與細胞毒性成反比。 使用酶标儀在450 nm 波長處測得吸光值,該測定值可間接反映活細胞的數量。本試劑盒的CCK-8 Solution 為即用型試劑, 可直接加入到細胞樣品中,孵育一定時間後即可檢測,不需要預配各種成分。

CCK-8 Cell Counting Kit 1000 rxn

價格:¥

貨号:A311-02
品牌:Vazyme

經銷:南京諾唯贊生物

CCK-8 Cell Counting Kit, 簡稱CCK-8 試劑盒, 是依賴于WST-8 (2-(2- 甲氧基-4- 硝苯基)-3-(4- 硝苯基)-5-(2,4- 二磺基苯)-2H- 四唑單鈉鹽) 而廣泛應用于細胞增殖和細胞毒性檢測的快速、高靈敏度、無放射性的比色檢測試劑盒。WST-8 在電子耦合載體1-Methoxy PMS 的作用下可被線粒體内的一些脫氫酶還原成高度水溶性的橙黃色甲臜産物(Formazan),生成的甲臜的顔色深淺與細胞增殖成正比,與細胞毒性成反比。 使用酶标儀在450 nm 波長處測得吸光值,該測定值可間接反映活細胞的數量。本試劑盒的CCK-8 Solution 為即用型試劑, 可直接加入到細胞樣品中,孵育一定時間後即可檢測,不需要預配各種成分。

ToxiLight™細胞毒性分析試劑盒(500次反應,有闆):ToxiLight™ BioAssay Kit 500次反應

價格:¥

貨号:LT17-217
品牌:Lonza

經銷:基因生物技術國際貿易(上海)有限公司

Eu-TDA細胞毒試劑盒:DELFIA® EuTDA Cytotoxicity Reagents 10闆*96

價格:¥

貨号:AD0116
品牌:PerkinElmer

經銷:基因生物技術國際貿易(上海)有限公司

Eu-Brdu細胞增殖試劑盒:DELFIA®Cell proliferation kit 10闆*96

價格:¥

貨号:AD0200
品牌:PerkinElmer

經銷:基因生物技術國際貿易(上海)有限公司

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