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生物通商城首頁 > 試劑 > 細胞生物學 > 信号通路檢測 > 抑制劑/激活劑

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Alpha SureFire磷酸化分析

細胞組分純化

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熒光素酶報告基因系統

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不限抑制劑
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不限 CST Santa Cruz 醫諾生物 NEB
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分類簡介:    高質量的化學激活劑和抑制劑與激活狀态和總蛋白抗體配合使用,對于探測關鍵信号節點和通路非常有用。
産品名/貨号/品牌 簡介 包裝  
原釩酸鈉(釩酸鹽):Sodium Orthovanadate (Vanadate), 1.0 ml Kit

價格:¥

貨号:P0758S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

Everolimus 1mg

價格:¥

貨号:12017S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

依維莫司,亦稱作RAD001,與FKBP12結合形成複合體,結合并抑制mTORC1活性。
Everolimus, 1mg

Paclitaxel 1mg

價格:¥

貨号:9807S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

紫杉醇屬于抗腫瘤和抗白血病藥物紫杉烷家族(3)。通過結合β-微管蛋白和促進微管裝配,紫杉醇可以防止微管解聚,并阻止正常細胞分裂(1-3)。紫杉醇引起的微管功能障礙能夠導緻異常的細胞有絲分裂和偶發的凋亡。紫杉醇誘導有絲分裂阻遏的IC50是4nM (4)。Paclitaxel為1mg粉末。-20ºC或更低溫度保存。使用前用1.15 ml DMSO稀釋溶解至1 mM貯存液。工作濃度為100 nM-1000 nM,将稀釋DMSO貯存液1:10,000 到 1:1000。用需要的濃度處理細胞6-48小時。Pacli
1 mg. Paclitaxel belongs to the taxane family of antitumor and antileukemic agents (3). By binding to β-tubulin and promoting the assembly of microtubules, paclitaxel prevents microtubual depolymerization and blocks normal cell division (1-3). The microtubule dysfunction induced by paclitaxel results in aberrant cell mitosis and sometimes apoptosis. The IC50 of paclitaxel-induced mitotic block is 4 nM (4).
Paclitaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1.15 ml DMSO to make a 1 mM paclitaxel stock solution. For working concentrations of 100 nM-1000 nM, dilute DMSO stock 1:10,000 to 1:1000. Treat cells with the desired concentration for 6-48 hours. Paclitaxel is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Tyrphostin AG 1478 400µg

價格:¥

貨号:9842S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

酪氨酸磷酸化抑制劑AG1478是一種特别選擇性抑制EGFR (ErbB1)的酪氨酸激酶抑制劑,體外實驗中,IC50值為3 nM(1,2)。用50-150 nM酪氨酸磷酸化抑制劑AG1478處理細胞可以充分阻斷EGFR在體内的激活(3)。除了EGFR, AG 1478還能夠抑制癌細胞因輻射引起的ErbB4激活(4)。AG 1478無論是單獨使用,還是聯合其他處理方式,在評價其抗腫瘤和抗纖維化的效果中都表現出不錯的應用前景。酪氨酸磷酸化抑制劑AG1478以400μg幹粉形式供應。存儲于-20ºC或更低的溫度下
400 µg. Tyrphostin AG 1478 is a tyrosine kinase inhibitor specifically selective to EGFR (ErbB1), with an IC50 of about 3 nM in vitro (1,2). Treatment of cell with 50-150 nM of AG 1478 can substantially block EGFR activiation in vivo (3). In addition to EGFR, AG 1478 also inhibits ErbB4 activation induced by radiation in cancer cells (4). Testing of AG 1478 alone or in combination with other treatments to assess anti-tumor and anti-fibrotic effectiveness has yielded promising results (5-8).
Tyrphostin AG 1478 is supplied as 400 μg powder. Store at or below -20ºC. Before use, dissolve powder in 1.23 ml DMSO to make a 1 mM 
 Tyrphostin AG 1478 stock solution. For working concentrations of 100 nM-200 nM, dilute DMSO stock 1:10,000 to 1:5,000. Treat cells with the desired concentration for 30 minutes. Tyrphostin AG1478 is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

Roscovitine 1mg

價格:¥

貨号:9885S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

核抑制劑是一種能透過細胞的周期蛋白依賴性激酶CDK1 (cdc2),CDK2和CDK5的選擇性抑制劑(1)。作為一種嘌呤類似物,該藥物競争性結合催化裂口的ATP位點。使用roscovitine處理培養的細胞能夠引起細胞周期捕獲或者凋亡(1-4)。該藥對于cdc2活性的體外IC50是0.65 μM . Roscovitine [(R) stereoisomer] 為1mg粉末。-20ºC或更低溫度保存。使用前用143 μl DMSO或MeOH稀釋溶解至20mM貯存液。推薦工作濃度為組織培養液中20 μM 。
1 mg. Roscovitine is a cell permeable reversible selective inhibitor of cyclin-dependent kinases CDK1 (cdc2), CDK2 and CDK5 (1). A purine analog, this drug competes for the binding site of ATP in the catalytic cleft. Treatment of cultured cells with roscovitine can cause cell cycle arrest or apoptosis (1-4). The IC50 for cdc2 activity is 0.65 μM in vitro (1).
Roscovitine [(R) stereoisomer] is supplied as a 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 143 μl DMSO or MeOH to make a 20 mM stock solution. The suggested working concentration is 20 μM in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Docetaxel 1mg

價格:¥

貨号:9886S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

多烯紫杉醇屬于抗腫瘤和抗白血病藥物的紫杉醇家族(1)。作為一種微管穩定藥物,多烯紫杉醇能夠充當需要微管動力學如細胞分裂的抑制劑,現在用于乳腺癌、前列腺癌和肺癌的治療(2)。Docetaxel為1mg粉末。-20ºC或更低溫度保存。使用前用1ml DMSO稀釋溶解至1mg/ml貯存液。推薦工作濃度為組織培養液中含100ng/ml。處理細胞4-24小時,貯存于-20ºC。
1 mg. Docetaxel belongs to the taxane family of antitumor and antileukemic agents (1). A microtubule-stabilizing agent, docetaxel acts as an inhibitor of cellular functions that require microtubule dynamics including cell division, and is now in use as a treatment for breast, prostate and lung cancer (2).
Docetaxel is supplied as 1 mg powder. Store at or below -20ºC. Before use, dissolve powder in 1 ml DMSO to make a 1 mg/ml stock solution. The suggested working concentration is 100 ng/ml in tissue culture medium. Treat cells for 4-24 hours. Store solution at -20ºC.

Vorinostat (SAHA) 5mg

價格:¥

貨号:12520S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

5 mg. MW=264.32 g/mol. C14H20N2O3. >99%. Vorinostat (SAHA) is a histone deacetylase (HDAC) inhibitor that acts by binding to and blocking the active site of the enzyme (1). Both class I and class II HDACs are inhibited by vorinostat at nanomolar concentrations (1-3). Research studies have shown that vorinostat causes the accumulation of acetylated histones, certain transcription factors, and other nonhistone proteins, which regulate gene expression and protein function. This leads to cell cycle arrest, differentiation, and/or apoptosis in many transformed cell types treated with micromolar concentrations of vorinostat. Vorinostat is supplied as a lyophilized powder. For a 20 mM stock, reconstitute the 5 mg in 945.8 µl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 2-24 hr. Soluble in DMSO at 66 mg/ml; soluble in ethanol at 2 mg/ml with slight warming; very poorly soluble in water with maximum solubility ~20-50 µM.

Rapamycin 10nmol

價格:¥

貨号:9904S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

Rapamycin(雷帕黴素)是一種具有抗真菌和免疫抑制活性的細菌大環内酯(1)。Rapamycin與親免素FKBP12形成複合物,從而抑制FRAP/ mTOR的活性(酵母中的TOR)(2,3)。雷帕黴素處理細胞後,導緻p70 S6激酶去磷酸化并失活。雷帕黴素也導緻4E-BP1/PHAS1去磷酸化,從而促進其與eIF4E結合并抑制其活性(4,5)。這個特性已被證明是雷帕黴素阻止蛋白質合成,及使細胞周期停滞在G1期的基礎(6,7)。然而,也有人認為雷帕黴素抑制G1 / S期轉換可能是由于其對cyclin D
10 nmol. Rapamycin is a bacterial macrolide with antifungal and immunosuppressant activities (1). Rapamycin forms a complex with the immunophilin FKBP12 which then inhibits the activity of FRAP/ mTOR (TOR in yeast) (2,3). Rapamycin treatment of cells leads to the dephosphorylation and inactivation of p70 S6 kinase. Rapamycin also leads to the dephosphorylation of 4E-BP1/PHAS1, thereby promoting its binding to and inactivation of eIF4E (4,5). This activity has been shown to be the basis for Rapamycin's ability to block protein synthesis and to arrest cell cycle progression in the G1-phase (6,7). However, it has been suggested that Rapamycin's inhibition of the G1/S transition may be the consequence of its effect on cyclin D1 mRNA and protein stability (8).
Rapamycin is supplied as a lyophilized powder. For a 100 µM stock, resuspend 10 nmol (9.1 µg) in 100 µl methanol or DMSO. Rapamycin is soluble in DMSO and methanol. For experiments with cultured cells, CST recommends pretreating with 10 nmol of this inhibitor for one hour prior to stimulation.

LY294002 1.5mg

價格:¥

貨号:9901S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

LY294002在體内是一個高度特異性的PI3K激酶抑制劑。在50 uM 濃度下,它可以特異地抑制 PI3K激酶的活性(IC50=0.43 μg/ml; 1.40 μM),但是不抑制其他的脂質和蛋白激酶活性,比如PI4 kinase, PKC, MAP kinase 或 c-Src (1)。 LY294002可溶解在DMSO或乙醇中。體外實驗或着用于細胞實驗時,可以将其稀釋于緩沖液中以得到所需的濃度。用于培養的細胞時,CST建議您用LY294002預處理1h,直到刺激結束。研究表明LY294002可以抑制
1.5 mg. LY294002 (PI3 Kinase Inhibitor) was shown to act in vivo as a highly selective inhibitor of phosphatidylinositol 3 (PI3) kinase. When used at a concentration of 50 μM, it specifically abolished PI3 kinase activity (IC50=0.43 μg/ml; 1.40 μM) but did not inhibit other lipid and protein kinases such as PI4 kinase, PKC, MAP kinase or c-Src (1). LY294002 is soluble in DMSO or ethanol. For use with in vitro or cell-based assays, it may be diluted into aqueous buffers to yield the desired concentrations. For experiments with cultured cells, CST recommends treating the cells with LY294002 for one hour prior to, and for the duration of, the stimulation. LY294002 has been shown to block PI3 kinase-dependent Akt phosphorylation and kinase activity.
For 10 mM stock, reconstitute 1.5 mg in 488 µl DMSO. For 50 mM stock, reconstitute 1.5 mg in 98 µl DMSO. Store aliquots at -20ºC.

PD98059 1.5mg

價格:¥

貨号:9900S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

PD98059 (MEK1 抑制子)是MEK1 活化和MAP激酶級聯反應的高選擇性抑制子(1-4)。PD98059結合MEK1的非活化形式,并阻止上遊活化因子如c-Raf的活化作用 (3)。本産品為凍幹的黃色粉末。配置20 mM儲存品時,取1.5 mg加入到280 µl DMSO 中(5 mg溶于933.3 μl體積中),配置50 mM儲存品時,取1.5 mg加入到112 µl DMSO 中(5 mg溶于373.3 μl體積中).分裝并儲存于-20ºC,詳細信息請查看附錄中的MSDS。該産品隻用于體内研究
1.5 mg(s) or 5 mg(L). PD98059 (MEK1 Inhibitor) has been shown to act in vivo as a highly selective inhibitor of MEK1 activation and the MAP kinase cascade (1-4). PD98059 binds to the inactive forms of MEK1 and prevents activation by upstream activators such as c-Raf (3).
Supplied as a lyophilized yellow powder. For 20 mM stock, reconstitute 1.5 mg in 280 µl DMSO (5 mg in 933.3 μl). For 50 mM stock, reconstitute 1.5 mg in 112 µl DMSO (5 mg in 373.3 μl). Store in aliquots at -20ºC. See enclosed MSDS for further information. This product is for in vitro research use only and is not intended for use in humans or animals.

Dasatinib 5mg

價格:¥

貨号:9052S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

達沙替尼是一種Src和Bcr-Abl酪氨酸激酶的小分子抑制劑,針對這兩種激酶的IC50值分别為0.55和3.0nm(1)。伊馬替尼和達沙替尼有相似的分子機制,但是達沙替尼對伊馬替尼耐藥的細胞系有較強的作用,并且對血小闆衍生生長因子(PDGF)有較強的抑制作用.提供的達沙替尼為凍幹粉。對于5mM濃度貯存液,在5mg凍幹粉中加入2.05 ml DMSO。工作濃度和處理時間根據所需的預期效果來确定,一般是50-500 nM,處理2-6小時。能溶于DMSO(200 mg/ml),難溶于乙醇和水,在水中的最大溶解度
>99% 5 mg.C22H26ClN7O2S. Dasatinib is a small molecule inhibitor of both the Src and Bcr-Abl tyrosine kinases, with IC50 values of 0.55 and 3.0 nM for the isolated kinases, respectively (1). Imatinib and dasatinib have similar inhibitory mechanisms, but dasatinib displays greater potency in imatinib-resistant cell lines and greater inhibitory effect on platelet derived growth factor (PDGF) (2,3).
Dasatinib is supplied as a lyophilized powder. For a 5 mM stock, reconstitute the 5 mg in 2.05 mL DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 50-500 nM for 2-6 hours. Soluble in DMSO at 200 mg/mL; very poorly soluble in ethanol and water with maximum solubility in water estimated to be about 10 μM.

Imatinib 5mg

價格:¥

貨号:9084S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

伊馬替尼是一種酪氨酸激酶(TK)抑制劑,是Bcr-Abl,PDGF受體和c-Kit TKs的特異性ATP結合位點拮抗物(1-3)。伊馬替尼在慢性髓細胞樣白血病(CML)的臨床試驗中結果很不錯,已經成為有針對性的癌症治療的範例(4-6)。利用磷酸化酪氨酸通路的信号傳導已經被廣泛研究,酪氨酸磷酸化與許多細胞生長和分化途徑有關(7-9)。因為觀察到的CML的狀态依賴于完整的Bcr-Abl酪氨酸激酶活性,我們做了多方面的工作來确認Bcr-Abl的底物和引起髓系分化的可能機制。許多研究小組已經發現了CML胚細胞和B
>99% 5 mg. C29H31N7O + CH4SO3. Imatinib is a tyrosine kinase (TK) inhibitor that is a relatively specific ATP-binding site antagonist of Bcr-Abl, PDGF receptor, and c-Kit TKs (1-3). Results are encouraging in chronic myeloid leukemia (CML) clinical trials and imatinib has become a paradigm for targeted cancer therapeutics (4-6). Signal transduction through phospho-tyrosine pathways has been studied extensively, and tyrosine phosphorylation has been linked to multiple cell growth and differentiation pathways (7-9). Because the observed leukemic state of CML is dependent on the intact Bcr-Abl tyrosine kinase activity, extensive work has been done to identify substrates of Bcr-Abl and thus possible mechanisms leading to a myeloid expansion. Many groups have characterized prominent tyrosine-phosphorylated protein substrates in both CML blasts and Bcr-Abl-expressing cell lines, including SHIP, c-Cbl, Dok, Shc, and CrkL (10-15). In addition, key signal transduction pathways involving PI3 kinase, Ras, Myc, and Stat5 are also activated in a Bcr-Abl kinase-dependent manner (16).
Imatinib is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 847.9 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 μM for 1-2 hours. Soluble in DMSO at 100 mg/ml; poorly soluble in ethanol. Soluble in water at 200 mg/ml.

MG-132 1mg

價格:¥

貨号:2194S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

反應種屬:Human

蛋白酶體和鈣蛋白酶抑制劑
MG-132, 1mg

PX-866 1mg

價格:¥

貨号:13055S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

Bortezomib 2.5mg

價格:¥

貨号:2204S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

硼替佐米(C19H25BN4O4)——硼酸二肽,是一種特異的、有效的、可逆的蛋白酶抑制劑,對抑制細胞存活和細胞周期進程的蛋白質有一個穩定效應,如p53(1,2)。研究者發現硼替佐米能抑制NF-κB的活化和核轉運,從而減少早期腫瘤存活(3)。能幹擾細胞存活和其他重要通路的、硼替佐米穩定的、錯誤折疊的、泛素化的蛋白的增加能進一步加強它的抗腫瘤作用. 提供的硼替佐米為凍幹粉。對于1mM濃度貯存液,在2.5mg凍幹粉中加入6.51ml DMSO。工作濃度和處理時間根據所需的預期效果來确定,一般是1-1000nM
>99% 2.5 mg.C19H25BN4O4. Bortezomib (C19H25BN4O4), a boronic acid dipeptide, is a specific, potent, and reversable proteasome inhibitor that has also been shown to have a stabilizing effect on proteins that inhibit cell survival and cell cycle progression, such as p53 (1,2). Researchers have demostrated that bortezomib inhibits activation and nuclear translocation of NF-κB, subsequently decreasing early tumor survival (3). The increase in bortezomib-stablilized, misfolded, and ubiquitinated proteins that interfere with cell survival and other important pathways further support its anti-tumor effects
Bortezomib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 6.51 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-1000 nM for 2-48 hours. Soluble in DMSO and ethanol at 200 mg/ml, very poorly soluble in plain water.

Crizotinib 2.5mg

價格:¥

貨号:4401S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

克唑替尼也被稱為PF-02341066,是一種新型的ATP競争性受體激酶抑制劑,對c-Met和間變性淋巴瘤激酶(ALK)等超過120多樣激酶顯示出較高的特異性(1,2)。研究人員證明,克唑替尼抑制c-Met的磷酸化和c-Met依賴性增殖,體外人體腫瘤細胞的遷移和侵襲(IC50值分别為5-20nM)(1)。克唑替尼對抗組成性激活的緻癌融合蛋白核磷蛋白(NPM)-ALK,抑制其磷酸化(平均IC50為24 nM),抑制細胞生長,在ALK-陽性ALCL (間變性大細胞淋巴瘤) 細胞系KARPAS-299和SU-D
>99% 2.5 mg. C21H22Cl2FN5O. Crizotinib, also known as PF-02341066, is a novel, ATP-competitive receptor kinase inhibitor, showing high specificity for c-Met and anaplastic lymphoma kinase (ALK) over 120 other diverse kinases (1,2). Researchers have shown that crizotinib inhibits c-Met phosphorylation and c-Met-dependent proliferation, migration, and invasion of human tumor cells in vitro (IC50 values of 5–20 nM) (1). Crizotinib is effective against the constitutively active oncogenic fusion protein nucleophosmin (NPM)-ALK, inhibiting its phosphorylation (mean IC50 of 24 nM), inhibiting cell growth, and inducing G1-S phase cell cycle arrest and apoptosis in the ALK-positive ALCL (anaplastic large-cell lymphoma) cell lines KARPAS-299 and SU-DHL-1
Crizotinib is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 2.5 mg in 5.55 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µM for 2-6 h. Soluble in DMSO at 25 mg/ml with warming; very poorly soluble in water with maximum solubility ~10-20 µM.

Doxorubicin 5mg

價格:¥

貨号:5927S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

多柔比星,一種蒽環類抗生素,能抑制哺乳動物中DNA和RNA的合成,也是一種非常有效的抗腫瘤藥物(1,2)。多柔比星通過嵌入DNA雙螺旋和穩定拓撲異構酶II裂解複合物來結合到核酸,導緻DNA在特異的多柔比星誘導的位點發生絲條斷裂(3)。在人乳腺癌細胞(MCF7)中,多柔比星能通過一種劑量依賴的方式抑制DNA合成,與生長抑制相符合(4)。研究者還發現多柔比星能夠有效抑制人DNA拓撲異構酶I(5)。提供的多柔比星為凍幹粉。對于10 mM濃度貯存液,在5mg凍幹粉中加入860 µl DMSO。工作濃度和處理時間根
>99% 5 mg. C27H29NO11 • HCl . Doxorubicin, an anthracycline antibiotic, inhibits DNA and RNA synthesis in mammalian cells and has been shown to be a very effective anti-tumor agent (1,2). Doxorubicin binds to nucleic acids by intercalating the DNA double helix and stabilizing topoisomerase II cleavage complexes, leading to DNA strand breaks at specific doxorubicin-induced sites (3). Doxorubin has been shown to inhibit DNA synthesis in a dose-dependent manner in MCF7 cells, which corresponds closely with growth inhibition (4). Researchers have also demonstrated that doxorubicin effectively inhibits human DNA topoisomerase I (5).
Doxorubicin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 860 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-5 µM for 12-24 hours. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol; soluble in water at 10 mg/ml with slight warming.

Cyclosporin A 100mg

價格:¥

貨号:9973S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

本産品的包裝規格是100mg粉末。-20ºC保存。Cyclosporin A粉末能夠溶解在乙醇(或者DMSO)中而配制成母液,母液也需要在-20ºC保存。工作液的濃度和處理時長可根據不同的實驗目的而變化,通常工作液的濃度約為100 nM。鈣調磷酸酶是cyclosporin A (CsA) (3) 和 FK-506 (又叫他克莫司和藤黴素)兩個免疫抑制劑的重要靶點,cyclosporin A (CsA)和 FK-506 能夠抑制抗原和分裂素對T細胞的激活,這兩個藥物分别與親免疫因子cyclophilin和F
100 mg。 Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models
Cyclosporin A is supplied as a 100 mg powder. Store at -20ºC. Cyclosporin A is soluble in ethanol (also DMSO) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect but it is typically used at around 100 nM.

Calyculin A 10µg

價格:¥

貨号:9902S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

花萼海綿誘癌素A絲氨酸/蘇氨酸抑制劑)與岡田軟海綿酸相比較,是一種更加有潛力的磷酸酶抑制劑(2)。Western blot實驗結果顯示,用100nM花萼海綿誘癌素A處理細胞30分鐘後,采用蘇氨酸磷酸化多克隆抗體#9381可以檢測到蘇氨酸發生磷酸化。對抗PP1的抑制活性IC50值大約為2nM。對抗PP2A的抑制活性IC50值大約為0.5-1.0nM。岡田軟海綿酸僅降低PP2A的活性而對PP1的活性幾乎沒有影響,與其不同的是花萼海綿誘癌素A可以抑制這兩種磷酸酶(1)。花萼海綿誘癌素A和岡田軟海綿酸都不能抑制酸
10 µg. Calyculin A (Serine/Threonine Phosphatase Inhibitor) is a more potent phosphatase inhibitor than Okadaic acid (2). As shown by Western blot, treatment of cells with 100 nM Calyculin A for 30 minutes induces threonine phosphorylation, detected by Phospho-Threonine-Polyclonal Antibody #9381. IC50 values for inhibitory activity against PP1 are approximately 2 nM. IC50 values for inhibitory activity against PP2A are approximately 0.5 -1.0 nM. Calyculin A inhibits the activity of protein phosphatases PP1 and PP2A (1,2). Unlike Okadaic acid, which reduces PP2A activity but has little effect on PP1 activity, Calyculin A inhibits both phosphatases (1). Neither Calyculin A nor Okadaic acid inhibit acid or alkaline phosphatases or phospho- tyrosine protein phosphatases (2).

Bisindolylmaleimide I, Hydrochloride 500µg

價格:¥

貨号:9841S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

Bisindolylmaleimide I(BIS)是PKC的強力抑制劑(1,2)。BIS在體外試驗的IC50,對PKCα/β/γ是10-20nM,對PKCδ/ε是100-200nM。在體外對PKCζ的IC50是6uM,表明BIS是此亞型非常弱的抑制劑。體内細胞分析BIS對PKC的IC50在0.2-2uM(1,3)。Bisindolylmaleimide I 以500ug粉劑提供。儲存在-20℃。使用前,将粉末溶解于0.28ml DMSO中産生4mM原液。對于工作濃度為2uM-4uM,将原液按1:2000
500 µg. Bisindolylmaleimide I (BIS) is a potent inhibitor of PKC (1,2). In vitro the IC50 of BIS is 10-20 nM for PKCα/β/γ and 100-200 nM for PKCδ/ε isoforms. The in vitro IC50 for PKCζ is about 6 μM, indicating that BIS is a very weak inhibitor for this isoform. In in vivo cellular assays the IC50 of BIS for PKC is between 0.2-2 μM (1,3).
Bisindolylmaleimide I is supplied as 500 μg powder. Store at or below -20ºC. Before use, dissolve powder in 0.28 ml DMSO to make a 4 mM bisindolylmaleimide I stock solution. For working concentrations of 2 μM-4 μM, dilute DMSO stock 1:2000 to 1:1000. Treat cells with the desired concentration for 30 minutes. Bisindolylmaleimide I is more stable as a powder than in solution; it is therefore recommended to dissolve as close as possible prior to use. Store solution at or below -20ºC.

TPA (12-O-Tetradecanoylphorbol-13-Acetate) 1mg

價格:¥

貨号:4174S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate)是最常用的佛波酯。它結合并激活蛋白激酶C,對很多細胞和組織産生影響(1,2)。TPA已被證明在在小鼠皮膚上是一個強有力的促癌物(3)。相反,它已被證明是粒細胞白血病的一種有效的治療藥物,在大腸癌治療中也有潛在療效(4-5)。TPA也被證明能在實質性癌症患者中增加白細胞和中性粒細胞(6)。TPA以1mg粉末提供。儲存在-20℃。使用前,将粉末溶解
1 mg. TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).
TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes.

Vatalanib 5mg

價格:¥

貨号:12998S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

瓦他拉尼,5mg,多靶酪氨酸酶抑制劑
Vatalanib,5mg

Lipopolysaccharides (LPS) 10mg

價格:¥

貨号:14011S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

10 mg. Lipopolysaccharide (LPS), also known as endotoxin, is a major glycolipid constituent of the outer cell wall of gram-negative bacteria. LPS molecules typically consist of a strain-specific distal polysaccharide side chain known as the O-antigen, a hydrophilic core oligosaccharide, and a hydrophobic domain referred to as lipid A. Lipid A is covalently bound to the outer bacterial membrane and is responsible for the toxicity of LPS (1-3). LPS is a potent activator of the proinflammatory response in many mammalian cell types, including macrophages, monocytes, and endothelial cells. Investigators have demonstrated that LPS binds to the CD14/TLR4/MD2 receptor complex, which in turn induces inflammatory cytokines including TNF-α, Interleukin-1, and IFN-α, as well as numerous inflammatory proteins such as iNOS, NF-κB, RIG-1, and IRF-3
For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml sterile PBS. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 µg/ml for 15 min-24 hours. Soluble in PBS and cell culture medium at 5 mg/ml and 1 mg/ml, respectively.

原釩酸鈉(釩酸鹽):Sodium Orthovanadate (Vanadate), 5.0 ml Kit

價格:¥

貨号:P0758L
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

Erlotinib 10mg

價格:¥

貨号:5083S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

厄洛替尼是一種新的和有效的EGFR激酶通路ATP競争性抑制劑。它在體外抑制EGFR的自磷酸化,并抑制純化的EGFR激酶,其IC50分别為20nM和2nM(1)。厄洛替尼對EGFR的選擇性比c-src和v-abl (1), ErbB-2和ErbB-4 (2)高出1000倍。有研究顯示,厄洛替尼在多種細胞類型中抑制生長并誘導G1細胞周期阻滞,這些細胞有許多是過量表達EGFR(1,3-5)。提供厄洛替尼的凍幹粉末。将其10mg重新溶解在2.54 ml DMSO中,即為10 mM儲存液。工作濃度和處理時間可以根據
>99% 10 mg. Erlotinib is a novel and potent ATP-competitive inhibitor of the EGFR kinase pathway. It inhibited EGFR autophosphorylation with an IC50 of 20 nM in vitro and inhibit purified EGFR kinase with an IC50 of 2 nM (1). Erlotonib is greater than 1000-fold more selective for EGFR than c-src and v-abl (1), ErbB-2, and ErbB-4 (2). Studies have shown that erlotinib inhibits growth and induces G1 cell cycle arrest in multiple cell types, many of which overexpress EGFR
Erlotinib is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.54 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used as a pretreatment at 0.1-10 µM for 0.5-2 hours prior to treating with a stimulator. It can also be used alone, with varying treatment times lasting up to 24 hours. Soluble in DMSO at 100 mg/ml, soluble in ethanol at 10 mg/ml with warming; very poorly soluble in water with a maximum solubility ~5-20 µM.

Nocodazole 10mg

價格:¥

貨号:2190S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

諾考達唑是一種抗腫瘤藥物,能可逆地幹擾微管的聚合(1)。它作為一種細胞周期同步化藥物廣泛用于細胞生物學實驗室,能夠誘導有絲分裂的停止,研究者已經證實高濃度的諾考達唑能誘導微管解聚,而低濃度時能改變紡錘體微管動力學,但微管不解聚(2-4)。最近的研究顯示諾考達唑是一種常用的多種癌症相關激酶的抑制劑,包括ABL, c-KIT, BRAF, MEK1, MEK2和MET。提供的諾考達唑為凍幹粉。對于1mg/ml濃度貯存液,在10 mg凍幹粉中加入10 ml DMSO。工作濃度和處理時間根據所需的預期效果來确定,
>97% 10 mg. C14H11N3O3S. Nocodazole is an anti-neoplastic agent that reversibly interferes with the polymerization of microtubules (1). Widely-used as a cell cycle synchronizing agent in cell biology labs to induce mitotic arrest, investigators have demonstrated that high concentrations of nocodazole induce microtubule depolymerization, whereas low concentrations alter spindle microtubule dynamics, but microtubules do not depolymerize (2-4). Recent research studies have demonstrated nocodazole to be a common inhibitor of various cancer-related kinases, including: ABL, c-KIT, BRAF, MEK1, MEK2, and MET
Nocodazole is supplied as a lyophilized powder. For a 1 mg/ml stock, reconstitute the 10 mg in 10 ml DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 0.1-1 µg/ml for 12-48 hr. Soluble in DMSO.

Etoposide 5.9mg

價格:¥

貨号:2200S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

依托泊苷(VP-16)是一種抗腫瘤劑,常用作凋亡誘導物,它還是一種拓撲異構酶II抑制劑(IC50為59.2 µM)(1)。依托泊苷能穩定酶催化性循環中的一種共價的拓撲異構酶II裂解的DNA中間複合物,導緻基因組不穩定性和細胞死亡(2,3)。這種作用機制能通過S晚期和G2早期來延誤細胞周期的進展. 提供的依托泊苷為凍幹粉。對于50 mM濃度貯存液,在5.9mg凍幹粉中加入200 µl DMSO。工作濃度和處理時間根據所需的預期效果來确定,一般是5-50 µM,處理4-24小時。能溶于DMSO(100 mg
>99% 5.9 mg. C29H32O13. An anti-tumor agent that is commonly used as an apoptosis inducer, etoposide (VP-16) is a topoisomerase II inhibitor with an IC50 of 59.2 µM (1). Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2,3). This mechanism of action has been shown to delay progression of the cell cycle through the late S and early G2 phase
Etoposide is supplied as a lyophilized powder. For a 50 mM stock, reconstitute the 5.9 mg in 200 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 4-24 hr. Soluble in DMSO at 100 mg/ml; very poorly soluble in ethanol and water with maximum solubility in water ~20-50 µM.

Brefeldin A 5mg

價格:¥

貨号:9972S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

Brefeldin A (BFA)是一個真菌代謝物,其被證明可逆地幹擾從内質網向高爾基體轉運(1,2)。當起初分離的作為一個抗生素(3),并且有一個廣泛的抗生素活性,它主要作為研究蛋白轉運的生物研究工具。BFA處理後導緻在内質網上一個快速的蛋白堆積,并且高爾基體會被破壞。BFA的處理也能抑制蛋白分泌(4)和延長的給藥時間能誘導凋亡(5)。BFA的主要目标出現是ADP-ribosylation factor (ARF),該蛋白複制對外殼蛋白到高爾基膜的關聯(6,7)。5 mg粉末。存儲-20ºC。Brefe
5 mg. Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulum to the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7).
Brefeldin A is supplied as a 5 mg powder. Store at -20ºC. Brefeldin A is soluble in DMSO (also ethanol and methanol) and stock solutions (typically 10 mg/ml) should be stored at -20ºC. Working concentrations and length of treatment can vary depending on desired effect. Inhibiton of ER to Golgi trafficking was observed as low as 100 ng/ml and apoptosis was observed with prolonged treatment at 10 μg/ml.

Okadaic Acid 25µg

價格:¥

貨号:5934S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

岡田酸是一種絲氨酸/蘇氨酸蛋白磷酸酶PP1 (IC50 = 15-20 nM)和PP2A (IC50 = 0.1 nM)的抑制劑,并且其對後者顯示出更高的活性。PP2A在1-2 nM就能被完全抑制,而PP1則要大于1 µM(1)。它與蛋白磷酸酶抑制劑花萼海綿誘癌素A不一樣,花萼海綿誘癌素A對PP1 (IC50 = 2 nM)和PP2A (IC50 = 0.5-1 nM)都能有效抑制(2)。岡田酸不能有效抑制PP2C(3),酸性和堿性磷酸酶以及磷酸酪氨酸蛋白磷酸酶(2,3)。它能抑制PP2B,但是所需濃度要
>98% 25 µg. C44H68O13. Okadaic acid is an inhibitor of the serine/theorine protein phosphatases PP1 (IC50 = 15-20 nM) and PP2A (IC50 = 0.1 nM), exhibiting much greater activity towards the latter. PP2A is completely inhibited at 1-2 nM, compared to greater than 1 µM for PP1 (1). This is unlike the protein phosphatase inhibitor Calyculin A, which potently inhibits both PP1 (IC50 = 2 nM) and PP2A (IC50 = 0.5-1 nM) (2). Okadaic acid does not effectively inhibit PP2C (3), acid and alkaline phosphatases, nor phosphotyrosine protein phosphatases (2,3). It has shown to inhibit PP2B, but at much higher concentrations than with PP1 and PP2A (3). Okadaic acid has also been described as a non-TPA type potent tumor promoter (4). These tumor-promoting properties and its ability to significantly increase protein phosphorylation make okadaic acid a very useful tool for studying cellular processes that are regulated by reversible phosphorylation of proteins (5).
Okadaic Acid is supplied as a lyophilized powder. For a 1 mM stock, reconstitute the 25 µg in 31.1 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 10-1000 nM for 15-60 min. Soluble in DMSO or ethanol.

FK-506 10mg

價格:¥

貨号:9974S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

FK-506的包裝規格是10mg粉末,在-20ºC保存。FK-506粉末能夠溶解在乙醇(或者DMSO和甲醇)中而配制成母液,母液需要在-20ºC保存。工作液的濃度和處理時長可根據不同的實驗目的而變化,通常工作液的濃度約在1~10nM之間。鈣調磷酸酶是cyclosporin A (CsA) 和 FK-506 (又叫他克莫司和藤黴素)兩個免疫抑制劑的重要靶點,cyclosporin A (CsA)和 FK-506 能夠抑制抗原和分裂素對T細胞的激活,這兩個藥物分别與親免疫因子cyclophilin和FKBP-
10 mg 。 Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models (6-8).
FK-506 is supplied as a 10 mg powder. Store at -20ºC. FK-506 is soluble in ethanol (also DMSO and methanol) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used between 1 and 10 nM.

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