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Animal-Free 封閉液(5X):Animal-Free Blocking Solution (5X) 25ml

價格:¥

貨号:15019S
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

Animal-Free 封閉液 (5X):Animal-Free Blocking Solution (5X) 250ml

價格:¥

貨号:15019L
品牌:CST

經銷:基因生物技術國際貿易(上海)有限公司

TBS Blotto A:TBS Blotto A 1 bottle

價格:¥

貨号:sc-2333
品牌:Santa Cruz

經銷:基因生物技術國際貿易(上海)有限公司

TBS Blotto A is supplied as a lyophilized powder in a single-use bottle; final reconstituted volume of 500 mLBlocking buffer and antibody diluent for Western blotting applications that contains Santa Cruz Biotechnology Immunoanalytical Grade Blottocontains 5% milk, 0.05% Tween-20; Tween-20 is included in a separate vial in liquid formmakes a 1x solution when reconstituted to the final volume of 500 ml in deionized H2OTBS Blotto A is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214For phospho-specific antibodies, we recommend our alternative product, TBS Blotto B: sc-2335 which contains a unique formulation of 1% milk, 1% blocking reagent, 0.05% Tween-20

TBS Blotto B:TBS Blotto B 1 bottle

價格:¥

貨号:sc-2335
品牌:Santa Cruz

經銷:基因生物技術國際貿易(上海)有限公司

TBS Blotto B 特别推薦用于磷酸化抗體, 以凍幹粉的形式裝在一次性使用的瓶中,重懸至終體積500 mL封閉試劑,以及一抗和二抗的稀釋液,可減少免疫印迹應用中的非特異結合和背景;優于BSA免疫分析級别的封閉液包含了1%奶粉, 1%封閉試劑, 0.05%吐溫-20; 吐溫-20以液态形式單獨裝在一個瓶子裡用去離子水重懸至500ml終體積,制備1X溶液為抑制絲氨酸/蘇氨酸磷酸酶活性, 可添加50mM 氟化鈉 (NaF)(sc-24988)到封閉液中.及/或 Sodium Orthovanadate (Na3O4V)(sc-3540)抑制蛋白質酪氨酸磷酸酶活性。另一種選擇, 可以添加磷酸酶抑制劑A(sc-45044)和磷酸酶抑制劑B(sc-45045)推薦用于降低總體的背景,減少與使用HRP标記的一抗、二抗,或者小鼠IgG結合蛋白的增強化學發光(ECL)蛋白檢測法相關的非特異信号。對于近紅外光譜(NIR)檢測法, 我們強烈推薦替代産品,UltraCruz® 封閉試劑 (sc-516214)如果不是用磷酸化抗體做免疫印迹,我們推薦替代産品,TBS Blotto A (sc-2333),它包含5%奶粉,0.05% 吐溫-20的獨特配方

Blotto, non-fat dry milk:Blotto, non-fat dry milk 250 g

價格:¥

貨号:sc-2324
品牌:Santa Cruz

經銷:基因生物技術國際貿易(上海)有限公司

Blotto, non-fat dry milk is supplied as a lyophilized powder in a single-use bottle; available in 2 sizes: 250 g and 500 gRecommended to prepare both our TBS Blotto A: sc-2333 and TBS Blotto B: sc-2335 blocking solutionsBlotto, non-fat dry milk is the preferred choice of blocking buffer and antibody diluent over BSA or alternative blocking buffers; strongly recommended to be used as an antibody diluent for both primary and secondary antibody incubations to reduce non-specific binding and backgroundRecommended to reduce overall background, and minimize non-specific signals commonly associated with enhanced chemiluminescent (ECL) protein detection methods using Horseradish Peroxidase (HRP) conjugated primary and secondary antibodies, or mouse IgG binding proteinsFor near-infrared (NIR) detection methods, we strongly recommend our alternative product, UltraCruz® Blocking Reagent: sc-516214

脫脂奶粉:Blotto, non-fat dry milk 500 g

價格:¥

貨号:sc-2325
品牌:Santa Cruz

經銷:基因生物技術國際貿易(上海)有限公司

UltraCruz Blocking Reagent:UltraCruz? Blocking Reagent, 100ml 1Unit

價格:¥

貨号:sc-516214
品牌:Santa Cruz

經銷:基因生物技術國際貿易(上海)有限公司

100 ml ready-to-use solution for blocking and dilutionPlant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications.animal-free alternative to sera, BSA or non-fat dry milkDesigned to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applicationsThe UltraCruz® Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are usedHighly recommended for use with Near-Infrared (NIR) Western blot detection methods, such as LI-COR/Odyssey® and other comparable systems

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