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生物通商城首頁 > 試劑 > 分子生物學 > 報告系統/蛋白标記定位 > SNAP-Surfase

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SNAP-Surface 阻斷劑 :SNAP-Surface Block, 200 nmol Kit

價格:¥

貨号:S9143S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

阻斷劑是非熒光底物,在活細胞表面(SNAP-Surface Block)阻斷 SNAP-tag 的反應。在 SNAP-tag 融合蛋白的活細胞标記實驗中,可以用阻斷劑制備無熒光對照。SNAP-Surface 阻斷劑也與 SNAP-tag 不可逆反應,使之在随後的标記步驟中失活。該阻斷劑不能透過細胞膜,僅可阻斷暴露于細胞表面的 SNAP-tag。

pSNAP-tag(T7)-2 載體:pSNAP-tag(T7)-2 Vector, 20 μg Kit

價格:¥

貨号:N9181S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the SNAP-tag. The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl chloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag. There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates.

pSNAPf 載體:pSNAPf Vector, 20 μg Kit

價格:¥

貨号:N9183S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

在哺乳動物細胞穩定和瞬時表達。 哺乳動物SNAPf載體表達各種SNAP-tags 的速度更快。tag 表達載體的上、下遊具有改良後的多克隆位點,使得 tag 可以表達在目的蛋白的任何一端,該表達過程受 CMV 啟動子調控。SNAPf-tag表達載體含有一個新黴素抗性基因(NeoR),可用于篩選穩定轉染子。載體上還帶有一個 IRES 元件,有助于融合蛋白和 NeoR 的高效表達。

SNAP-Surface 549, 50 nmol Kit

價格:¥

貨号:S9112S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光560nm,發射光575nm。SNAP-Surface™ 549 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate (BG-549) is based on the Dyomics dye DY-549P1 and is suitable for use with standard TAMRA or Cy3 filter sets. It has an excitation maximum at 560 nm and emission maximum at 575 nm. This package includes 50 nmol of SNAP-Surface 549, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface 488, 50 nmol Kit

價格:¥

貨号:S9124S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光506 nm,發射光526nm。SNAP-Surface® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate (BG-488) is based on ATTO-TEC dye ATTO 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 506 nm and emission maximum at 526 nm. This package includes 50 nmol of SNAP-Surface 488, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface 649, 50 nmol Kit

價格:¥

貨号:S9159S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發波長655nm,發射波長676nm。SNAP-Surface 649 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins on cell surfaces and in solution. This substrate (BG-649) is based on the Dyomics dye DY-649P1 and is suitable for excitation with a 650 nm diode laser or use with Cy5 filter sets. It has an excitation maximum at 655 nm and emission maximum at 676 nm. This package includes 50 nmol of SNAP-Surface 649 substrate, sufficient to make 10 ml of a 5 μM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine- DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAPtag substrate is described below.

SNAP-Surface 594, 50 nmol Kit

價格:¥

貨号:S9134S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光606nm,發射光626 nm。SNAP-Surface® 594 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living or fixed cells. This substrate (BG-594) is based on the ATTO-TEC dye ATTO 594 and is suitable for standard Texas Red and Alexa 594 filter sets. It has an excitation maximum at 606 nm and emission maximum at 626 nm. This package includes 50 nmol of SNAP-Surface 594 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a small protein based on manmmalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylpurines and benzylchloropyrimidines, In the labeling reaction, the substituted benzyl group of the substrate becomes covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface Alexa Fluor 647, 50 nmol Kit

價格:¥

貨号:S9136S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光652nm,發射光670nm。SNAP-Surface Alexa Fluor 647 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on Alexa Fluor 647 and is suitable for standard 635 nm or 650 nm diode laser excitation. It has an excitation maximum at 652 nm and emission maximum at 670 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 647 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface Alexa Fluor® 488:SNAP-Surface Alexa Fluor 488, 50 nmol Kit

價格:¥

貨号:S9129S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光496nm,發射光520nm。SNAP-Surface® Alexa Fluor® 488 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on BG-Alexa Fluor 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 496 nm and emission maximum at 520 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 488 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

SNAP-Surface Alexa Fluor 546, 50 nmol Kit

價格:¥

貨号:S9132S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(SNAP-Surface)僅能特異性的标記在細胞表面表達的融合蛋白。激發光 558nm,發射光574nm。SNAP-Surface Alexa Fluor 546 is a photostable fluorescent substrate that can be used to label SNAP-tag® fusion proteins in solution or on the surface of living cells. This cell impermeable substrate is based on Alexa Fluor 546 and is suitable for standard TAMRA or Cy3 filter sets. It has an excitation maximum at 558 nm and emission maximum at 574 nm. This package includes 50 nmol of SNAP-Surface Alexa Fluor 546 substrate, sufficient to make 10 ml of a 5 µM SNAP-tag fusion protein labeling solution.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on mammalian O6-alkylguanine-DNA-alkyltransferase (AGT). SNAP-tag substrates are derivatives of benzylguanines and benzylchloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub-cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

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