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CoA-Biotin, 50 nmol Kit

價格:¥

貨号:S9351S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

細胞非通透性底物(CoABiotin)通過一個氨己酰基與生物素相連。生物素标記物可用于對活細胞内部或表面的融合蛋白的生物素化,從而檢測鍊親和素熒光基耦聯物,或在溶液中标記,以進行 SDS-PAGE/ Western blot 分析。生物素标記物也被用于與鍊黴親和素結合,以進行結合和相互作用方面的研究。

ACP 合成酶 :ACP Synthase, 25 nmol Kit

價格:¥

貨号:P9301S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

ACP 合成酶 (4' -磷酸泛酰巯基乙胺基轉移酶) 催化輔酶 A (CoA) 上的取代基共價轉移至暴露于活細胞表面的 ACP-tag 融合蛋白。ACP Synthase (4´-phosphopantetheinyl transferase) catalyzes the covalent transfer of substituents from derivatized coenzyme A (CoA) to ACP-tagged fusion proteins exposed on the surface of living cells. The 25 nmoles of ACP Synthase provided is sufficient to make 25 ml of a 1 µM ACP-tag fusion protein labeling solution.
The ACP-tag and MCP-tag are small tags (8 kDa) based on the acyl carrier protein (ACP). MCP-tag contains two mutations (D36-T36 and D39-G39). Both allow the specific, covalent attachment of virtually any molecule to a protein of interest. Substrates for labeling are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue on the ACP-tag or the MCP-tag by a phosphopantetheine transferase (SFP Synthase or ACP Synthase). Having no cysteines, the ACP-tag and the MCP-tag are particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.
While ACP Synthase will preferentially modify the ACP-tag, SFP Synthase (NEB #P9302 ) will modify both ACP-tag and MCP-tag. This principle can be employed for sequential dual labeling of two different proteins that localize to the cell surface. Cells co-expressing one ACP-tag fusion protein and one MCP-tag fusion protein can be incubated with ACP Synthase and one CoA substrate followed by labeling with SFP Synthase and a different CoA substrate.
There are two steps to using this system: cloning and expression of the protein of interest as an ACP-tag fusion, and labeling of the fusion protein using the ACP Synthase with the CoA substrate of choice. In this document, the labeling of fusion proteins with CoA substrates is described. The cloning of ACP-tag protein fusions is described in the documentation supplied with the ACP-tag plasmids.

SFP 合成酶 :SFP Synthase, 25 nmol Kit

價格:¥

貨号:P9302S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

SFP 合成酶 (4' -磷酸泛酰巯基乙胺基轉移酶) 可以催化輔酶 A (CoA) 上的取代基共價連接到暴露于活細胞表面的 ACP- 或 MCP-tag 融合蛋白上。

CoA 488, 50 nmol Kit

價格:¥

貨号:S9348S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

激發光506 nm 發射光 526 nm. ACP/MCP-tag的标記基團與輔酶 A(CoA)的磷酸泛酰巯基乙胺耦聯,在 ACP 或 SFP 合成酶作用下,完成标記反應。标記反應僅特異地發生在細胞表面表達的融合蛋白上。雖然 ACP- 和 MCP-tag 使用相同底物,但是,反應特異性卻不同,表現在需要用不同的合成酶進行标記反應。CoA 488 is a photostable fluorescent substrate used to label ACP-tag and MCP-tag fusion proteins exposed on the surface of living cells. This cell-impermeable CoA substrate is based on the ATTO-TEC dye ATTO 488, and is suitable for standard fluorescein filter sets. It has an excitation maximum at 506 nm and an emission maximum at 526 nm. This package contains 50 nmol of CoA 488 substrate, sufficient to make 10 ml of a 5 μM ACP-tag or MCP-tag fusion protein labeling solution.

The ACP-tag and MCP-tag are small protein tags (8 kDa) based on the acyl carrier protein. MCP-tag contains two mutations (D36T and D39G). Both allow the specific, covalent attachment of virtually any molecule to a protein of interest. Substrates are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue of the ACP-tag or the MCP-tag by a phosphopantetheinyl transferase (SFP Synthase or ACP Synthase).

While ACP Synthase (NEB #P9301) will preferentially label the ACP-tag, SFP Synthase (NEB #P9302) will modify both ACP-tag and MCP-tag. Having no cysteines, the ACP-tag and the MCP-tag are particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.

There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag or MCP-tag fusion, and labeling of the fusion protein using the appropriate synthase with the CoA substrate of choice. Expression of ACP- and MCP-tagged proteins is described in the documentation supplied with the pACP-tag and pMCP-tag plasmids, respectively. The labeling of the fusion proteins with the CoA substrate is described below.

CoA 547, 50 nmol Kit

價格:¥

貨号:S9349S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

激發光554 nm 發射光 568 nm. ACP/MCP-tag的标記基團與輔酶 A(CoA)的磷酸泛酰巯基乙胺耦聯,在 ACP 或 SFP 合成酶作用下,完成标記反應。标記反應僅特異地發生在細胞表面表達的融合蛋白上。雖然 ACP- 和 MCP-tag 使用相同底物,但是,反應特異性卻不同,表現在需要用不同的合成酶進行标記反應。CoA 547 is a photostable fluorescent substrate that can be used to label ACP-tag and MCP-tag fusion proteins exposed on the surface of living cells. This cell-impermeable substrate is based on the Dyomics dye DY-547, and is suitable for standard TAMRA and Cy3 filter sets. It has an excitation maximum at 554 nm and an emission maximum at 568 nm. This package contains 50 nmol of CoA 547 substrate, sufficient to make 10 ml of a 5 μM ACP-tag or MCP-tag fusion protein labeling solution.

The ACP-tag and MCP-tag are small protein tags (8 kDa) based on the acyl carrier protein. MCP-tag contains two mutations (D36T and D39G). Both allow the specific, covalent attachment of virtually any molecule to a protein of interest. Substrates are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue of the ACP-tag or the MCP-tag by a phosphopantetheinyl transferase (SFP Synthase or ACP Synthase).

While ACP Synthase (NEB #9301) will preferentially label the ACP-tag, SFP Synthase (NEB #P9302) will modify both ACP-tag and MCP-tag. Having no cysteines, the ACP-tag and the MCP-tag are particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.

There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag or MCP-tag fusion, and labeling of the fusion protein using the appropriate synthase with the CoA substrate of choice. Expression of ACP- and MCP-tagged proteins is described in the documentation supplied with the ACP-tag and MCP-tag plasmids, respectively. The labeling of the fusion proteins with the CoA substrate is described below.

CoA 647, 50 nmol Kit

價格:¥

貨号:S9350S
品牌:NEB

經銷:基因生物技術國際貿易(上海)有限公司

激發光660 nm 發射光 673 nm. CoA 647 is a photostable fluorescent substrate that can be used to label ACP-tag or MCP-tag fusion proteins exposed on the surface of living cells. This cell-impermeable substrate is based on the Dyomics dye DY-647P1, and is suitable for Cy5 lasers. It has an excitation maximum at 660 nm and an emission maximum at 673 nm. The 50 nmol of CoA 647 in each vial is sufficient to make 10 ml of a 5 μM ACP-tag or MCP-tag fusion protein labeling solution.

The ACP-tag and MCP-tag are small protein tags (8 kDa) based on the acyl carrier protein. MCP-tag contains two mutations (D36T and D39G). Both allow the specific, covalent attachment of virtually any molecule to a protein of interest. Substrates are derivatives of coenzyme A (CoA). In the labeling reaction, the substituted phosphopantetheine group of CoA is covalently attached to a conserved serine residue of the ACP-tag or the MCP-tag by a phosphopantetheinyl transferase (SFP Synthase or ACP Synthase).

While ACP Synthase (NEB #P9301) will preferentially label the ACP-tag, SFP Synthase (NEB #P9302) will label both ACP-tag and MCP-tag. Having no cysteines, the ACP-tag and the MCP-tag are particularly suited for specifically labeling cell-surface proteins, and should be useful for labeling secreted proteins with disulfide bridges such as antibodies.

There are two steps to using this system: subcloning and expression of the protein of interest as an ACP-tag or MCP-tag fusion, and labeling of the fusion protein using the appropriate synthase with the CoA substrate of choice. Expression of ACP- and MCP-tagged proteins is described in the documentation supplied with the ACP-tag and MCP-tag plasmids, respectively. The labeling of the fusion proteins with the CoA substrate is described below.

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