收藏生物通商城 您好!歡迎來到生物通商城![請登錄],新用戶?[免費注冊] 立即咨詢 QQ 點擊這裡給我發消息 我的訂單 幫助

商品比較

生物通商城首頁 > 試劑 > 細胞生物學 > 融合蛋白标記定位 > CLIP-tag系統

細胞生物學

活體成像試劑

Alpha SureFire磷酸化分析

細胞組分純化

磷酸化蛋白純化

熒光素酶報告基因系統

融合蛋白标記定位

細胞染料/标記示蹤

增殖/活力/毒性分析

藥物代謝分析

細胞分析

細胞周期

細胞凋亡檢測

第二信使/cAMP/cGMP

細胞能量代謝分析

受體/膜研究

細胞因子/生長因子

細胞因子檢測

信号通路檢測

蛋白純品對照

熒光原位雜交

細胞成像分析

細胞計數

各類細胞(RNAlater保存/提取RNA)

細胞凍存

激酶研究

磷酸化研究

磷酸化酶富集

磷酸檢測

細胞分選

  • 促銷專題
  • 您浏覽過的産品

清除曆史

商品篩選

品牌:
不限 NEB
抗體篩選:
篩選:
簡介:
分類簡介:

SNAP-tag 和 CLIP-tag 蛋白标記系統能将目标蛋白以共價結合的形式,特異性地标記上任何分子。使用該系統時應包括兩個步驟:亞克隆目标蛋白質,使其與 SNAP-tag 形成融合表達蛋白;選擇理想底物标記SNAP-tag。SNAP-tag 是一種分子量較小的多肽,來自 DNA 修複蛋白人烷基鳥嘌呤-DNA 烷基轉移酶(O6-alkylguanine-DNA-alkyltransferase,hAGT),SNAP-tag的底物可以是通過苄基與鳥嘌呤或氯嘧啶基團偶聯的熒光基團、生物素或微珠。在标記反應中,底物的苄基部份被替換,與 SNAP-tag 形成共價結合。CLIP-tag 是 SNAP-tag 的改良版本,與苄基胞嘧啶反應,而不是與苄基鳥嘌呤的衍生物反應。CLIP-tag與 SNAP-tag 共同使用時,能在同一細胞中同時标記兩種蛋白。

 

CLIP-Cell™:CLIP-Cell 标記物是細胞通透性底物,适用于在活細胞内、固定細胞内、活細胞表面、固細胞表面或體外标記攜帶有 CLIP-tag 的融合蛋白。CLIPtag由 SNAP-tag 衍生而來,與不同底物特異性結合,可以同時以不同熒光底物标記兩種表達蛋白。該類産品的阻斷劑是具有細胞通透性的非熒光标記物。

 

CLIP-Surface™:CLIP-Surface 标記物是細胞非通透性底物,适用于在活細胞表面、固定細胞表面或體外标記攜帶有 CLIP-tag 的融合蛋白。CLIP-tag 由SNAP-tag 衍生而來,與不同底物特異性結合,可以同時以不同熒光底物标記兩種表達蛋白。這類标記物包括了常用可見光譜區域(如 488、547 和647 nm)的探針。

 

産品名/貨号/品牌 簡介 包裝  
CLIP-Cell 阻斷劑 :CLIP-Cell Block, 100 nmol Kit

價格:¥

貨号:S9220S
品牌:NEB

經銷:基因有限公司

非熒光底物,可順利透過細胞膜,在細胞内或活細胞表面阻斷SNAP-tag的反應,使之在後續的标記反應中不可逆地失活。在Clip-tag融合蛋白的活細胞标記實驗中,可以用阻斷劑制備無熒光對照

pCLIPf 載體:pCLIPf Vector, 20 μg Kit

價格:¥

貨号:N9215S
品牌:NEB

經銷:基因有限公司

CLIP-Surface 啟動試劑盒:CLIP-Surface Starter Kit, 1 set Kit

價格:¥

貨号:E9230S
品牌:NEB

經銷:基因有限公司

蛋白質标記系統啟動試劑盒提供了标記CLIP-tag融合蛋白的所有必需組份。能在活細胞、固定細胞及體外将紅色或綠色熒光基團共價連接到目标蛋白上。每種啟動試劑盒包括一個編碼所選 tag 的質粒和細胞非通透性的熒光标記物。試劑盒中還提供一種陽性對照質粒,其編碼有亞細胞定位明确的标簽蛋白(如:膜蛋白、核蛋白等)。如果必要,試劑盒還會提供一個與目的标簽相互作用的、非熒光的陰性對照阻斷劑。The CLIP-Surface Starter Kit contains a mammalian expression plasmid (pCLIPf) encoding the CLIP-tag flanked by restriction sites for cloning a gene of interest, and two non-cell-permeable fluorescent CLIP-tag substrates. A positive control plasmid (pCLIPf-NK1R), encoding a CLIP-tagged protein (neurokinin-1 receptor) with a well-characterized cell surface localization, is also included. Lastly, a negative control “blocking agent” (CLIP-Cell™ Block) is included that interacts with the CLIP-tag, but is not fluorescent. There are two steps to using this system: subcloning and expression of the protein of interest as a CLIPf fusion, and labeling of the fusion with the CLIP-tag substrate of choice.

The SNAP-tag® and CLIP-tag™ are novel tools for the specific, covalent attachment of virtually any molecule to a protein of interest, providing simplicity and extraordinary versatility to the imaging of proteins in live and fixed cells, and to the study of proteins in vitro. The creation of a single gene construct yields a tagged fusion protein capable of forming a covalent linkage to a variety of functional groups, including fluorophores, biotin, or beads. This system provides a powerful and unique tool to study the role of proteins in a variety of highly dynamic processes, including protein trafficking, turnover and complex formation.

The CLIP-tag is a 20 kDa mutant of the human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) that reacts specifically and rapidly with benzylcytosine (BC) derivatives, leading to covalent labeling of the CLIP-tag with a synthetic probe (Figure 1). The CLIP-tag has a number of features that make it ideal for a variety of protein labeling applications. The rate of the reaction of the CLIP-tag with these derivatives is largely independent of the nature of the synthetic probe attached to BC, permitting the labeling of CLIP fusion proteins with a wide variety of functional groups. Many of these CLIP-tag substrates are non-cell-permeable, allowing live-cell imaging of protein expression and localization on the cell surface (Figure 2). The ability to turn on the signal at will, together with the availability of a nonfluorescent blocking agent (CLIP-Cell™ Block), allows time-resolved pulse-chase analysis of protein trafficking to the cell surface, as well as subsequent internalization. Finally, the availability of orthogonal protein labeling systems from NEB permits simultaneous labeling of multiple proteins in a single cell (SNAP-tag, another hAGT variant that reacts exclusively with O6-benzylguanine substrates, and the ACP/MCP tags, small protein tags which can be enzymatically labeled on the cell surface with Coenzyme A derivatives).

CLIP-Cell 啟動試劑盒:CLIP-Cell Starter Kit, 1 set Kit

價格:¥

貨号:E9200S
品牌:NEB

經銷:基因有限公司

蛋白質标記系統啟動試劑盒提供了标記CLIP-tag融合蛋白的所有必需組份。能在活細胞、固定細胞及體外将紅色或綠色熒光基團共價連接到目标蛋白上。每種啟動試劑盒包括一個編碼所選 tag 的質粒和兩種細胞通透性的熒光标記物。試劑盒中還提供一種陽性對照質粒,其編碼有亞細胞定位明确的标簽蛋白(如:膜蛋白、核蛋白等)。如果必要,試劑盒還會提供一個與目的标簽相互作用的、非熒光的陰性對照阻斷劑。The SNAP-tag® and CLIP-tag™ are novel tools for the specific, covalent attachment of virtually any molecule to a protein of interest, providing simplicity and extraordinary versatility to the imaging of proteins in live and fixed cells, and to the study of proteins in vitro. The creation of a single gene construct yields a tagged fusion protein capable of forming a covalent linkage to a variety of functional groups, including fluorophores, biotin, or beads. This system provides a powerful and unique tool to study the role of proteins in a variety of highly dynamic processes, including protein trafficking, turnover and complex formation. The CLIP-tag is a 20 kDa mutant of the human DNA repair protein O6-alkylguanine- DNA alkyltransferase (hAGT) that reacts specifically and rapidly with benzylcytosine (BC) derivatives, leading to covalent labeling of the CLIP-tag with a synthetic probe (Figure 1).

The CLIP-tag has a number of features that make it ideal for a variety of protein labeling applications. The rate of the reaction of the CLIP-tag with these derivatives is largely independent of the nature of the synthetic probe attached to BC, permitting the labeling of CLIP fusion proteins with a wide variety of functional groups. Many of these CLIP-tag substrates are cell-permeable, allowing live-cell imaging of protein expression and localization (Figure 2). The ability to turn on the signal at will, together with the availability of a cell-permeable nonfluorescent blocking agent (CLIP-Cell™ Block), allows time-resolved pulse-chase analysis of protein trafficking. Finally, the availability of orthogonal protein labeling systems from NEB permits simultaneous labeling of multiple proteins in a single cell (SNAP-tag, another hAGT variant that reacts exclusively with O6-benzylguanine substrates, and the ACP/MCP tags, small protein tags which can be enzymatically labeled on the cell surface with Coenzyme A derivatives).

The CLIP-Cell Starter Kit contains a mammalian expression plasmid (pCLIPf) encoding the CLIP-tag flanked by restriction sites for cloning a gene of interest, and two cell-permeable fluorescent CLIP-tag substrates. A positive control plasmid (pCLIPf-H2B), encoding a CLIP-tagged protein (histone H2B) with a well-characterized nuclear localization, is also included. Lastly, a negative control “blocking agent” (CLIP-Cell Block) is included that interacts with the CLIP-tag, but is not fluorescent. There are two steps to using this system: subcloning and expression of the protein of interest as a CLIPf fusion, and labeling of the fusion with the CLIP-tag substrate of choice.

CLIP-Biotin, 50 nmol Kit

價格:¥

貨号:S9221S
品牌:NEB

經銷:基因有限公司

CLIP-Cell 505, 50 nmol Kit

價格:¥

貨号:S9217S
品牌:NEB

經銷:基因有限公司

細胞通透性底物(CLIP-Cell)激發波長504nm, 發射波長532nm。CLIP-Cell™ 505 is a green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro. This cell-permeable substrate (BC-505) is based on the Dyomics dye DY-505 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 504 nm and emission maximum at 532 nm. This package includes 50 nmol of CLIP-Cell 505 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.

The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNAalkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion with the CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins is described in this document.

CLIP-Cell TMR-Star, 30 nmol Kit

價格:¥

貨号:S9219S
品牌:NEB

經銷:基因有限公司

細胞通透性底物(CLIP-Cell)激發波長554nm, 發射波長580nm。CLIP-Cell™ TMR-Star is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins inside living cells, on cell surfaces or in vitro. This cell-permeable substrate (BC-TMR) is based on tetramethylrhodamine and is suitable for standard rhodamine filter sets. It has an excitation maximum at 554 nm and emission maximum at 580 nm. This package includes 30 nmol of CLIP-Cell TMR-Star, sufficient to make 10 ml of a 3 µM CLIP-tag fusion protein labeling solution.

The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNAalkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins with the CLIP-tag substrate is described in this document.

CLIP-Surface 488, 50 nmol Kit

價格:¥

貨号:S9232S
品牌:NEB

經銷:基因有限公司

細胞非通透性底物(CLIP-Surface)激發波長506nm, 發射波長526nm。CLIP-Surface™ 488 is a photostable green fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro. This cell impermeable substrate (BC-488) is based on ATTO-TEC dye ATTO 488 and is suitable for standard fluorescein filter sets. It has an excitation maximum at 506 nm and emission maximum at 526 nm. This package includes 50 nmol of CLIP-Surface 488 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag™ fusion protein labeling solution.

The CLIP-tag™ protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag, the benzylcytosine substrates form a separate class of substrates, different from the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag® can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.

CLIP-Surface 547, 50 nmol Kit

價格:¥

貨号:S9233S
品牌:NEB

經銷:基因有限公司

細胞非通透性底物(CLIP-Surface)激發波長554nm, 發射波長568nm。CLIP-Surface™ 547 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro. This cell impermeable substrate (BC-547) is based on the Dyomics dye DY-547 and is suitable for use with standard TAMRA or Cy3 filter sets. It has an excitation maximum at 554 nm and emission maximum at 568 nm. This package includes 50 nmol of CLIP-Surface 547 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.

The CLIP-tag™ protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag, the benzylcytosine substrates form a separate class of substrates, different from the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag® can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.

CLIP-Surface 647, 50 nmol Kit

價格:¥

貨号:S9234S
品牌:NEB

經銷:基因有限公司

細胞非通透性底物(CLIP-Surface)激發波長660nm, 發射波長673nm。CLIP-Surface™ 647 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells, or in vitro. This cell impermeable substrate (BC-647) is based on the Dyomics dye DY-647 and is suitable for 635 nm and 650 nm diode laser excitation or use with Cy5 filter sets. It has an excitation maximum at 660 nm and emission maximum at 673 nm. This package includes 50 nmol of CLIP-Surface 647 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.

The CLIP-tag™ protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag, the benzylcytosine substrates form a separate class of substrates, different from the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag® can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.

BC-NH2, 2 mg Kit

價格:¥

貨号:S9236S
品牌:NEB

經銷:基因有限公司

CLIP-tag底物,苄基胞嘧啶(benzylcytosine,BC),用于連接NHS酯和其他含活化羧基的酯。可用于将新的分子或配基連至蛋白質;制作用于标記蛋白的自定義底物;制作用于蛋白質固定的載體表面。如耦聯至 Biacore® 芯片或微陣列表面,以固定特定的蛋白質;還可耦聯至多肽、蛋白質或DNA寡聚物。耦聯至新的熒光基團或親和試劑,可以标記特定的蛋白質。标記反應溫和、精确且作用于多種底物:在生物學條件下,标記物可以在特定位置形成共價鍵。

第1 / 1頁 共11條 上一頁 下一頁

生物通 版權所有

未經書面授權,所有頁面内容、圖片和設計不得以任何形式進行複制

Copyright © 2000-2019 eBiotrade.com, All Rights Reserved

本欄目客服聯系電話:廣州 020-85524840轉1060

本欄目廣告聯系電話:廣州 020-87511980

主編信箱:

粵公網安備 44010602000434号 免責聲明

7ms 11ms 889ms 1031ms 1494ms 852 853
http://9fu8zk.dnshc4h.top| http://416c.dnshc4h.top| http://82hvdfgb.dnshc4h.top| http://f4b2.dnshc4h.top| http://tnd8sta.dnshc4h.top|