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SimpleDIP™ Methylated DNA IP (MeDIP) Kit 1Kit

價格:¥

貨号:76853S
品牌:CST

經銷:基因有限公司

SimpleDIP™甲基化DNA免疫沉澱試劑盒,應用MeDIP ;儲存溫度4℃ and -20℃;The SimpleDIP™ Methylated DNA IP (MeDIP) Kit can be utilized to detect endogenous levels of 5-methylcytosine modifications in mammalian cells (see Figures 1 and 2). The 5-Methylcytosine (5-mC) (D3S2Z) Rabbit mAb has been validated for specificity using ELISA, dot blot, and MeDIP assays and shows high specificity for its target DNA modification (see Figures 3-5). Positive control primer sets are included for human testis-specific H2B (TSH2B) promoter and mouse intracisternal A-particle (IAP) LTR, two genomic loci known to be methylated in most cell types. The use of other species with the kit requires the design of additional control primer sets.

SimpleDIP™ Hydroxymethylated DNA IP (hMeDIP) 1Kit

價格:¥

貨号:95176S
品牌:CST

經銷:基因有限公司

SimpleDIP™羟甲基化DNA免疫沉澱試劑盒;應用MeDIP ;儲存溫度4℃ and -20℃;
The SimpleDIP™ Hydroxymethylated DNA IP (hMeDIP) Kit provides enough reagents to perform up to 10 genomic DNA preparations and 10 IPs from mammalian cells and is optimized for 1 μg of genomic DNA per IP. The SimpleDIP™ protocol can be performed in as little as two days and can easily be scaled up or down for use with more or less cells. Cells are first lysed and genomic DNA is extracted and sonicated into small fragments (200-500 bp). DNA IPs are performed using 5-Hydroxymethylcytosine (5-hmC) (HMC31) Mouse mAb and ChIP-Grade Protein G Magnetic Beads. After elution from the beads, the DNA is purified using DNA purification spin columns provided in the kit. The enrichment of particular DNA sequences can be analyzed by a variety of methods including standard PCR, quantitative real-time PCR, or next-generation sequencing. The SimpleDIP™ 5-Hydroxymethylcytosine DNA IP Kit provides a highly validated 5-hmC monoclonal antibody to ensure specific and robust signal. The kit also includes DNA that contains exclusively 5-hydroxymethylcytosine, which can be spiked-in to the IPs as a control. Thus, spiked-in DNA will be immunoprecipitated with 5-Hydroxymethylcytosine (HMC1) Mouse mAb, but not with the Mouse (G3A1) mAb IgG1 Isotype Control (DIP Formulated). The relative enrichment can then be quantified using the SimpleDIP Hydroxymethyl Control Primers.

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