微信下單
全部分類

最新加入的商品

          • 0件商品
            共計:¥0.00
          共計¥0.00
          去購物車
          • 促銷專題
          生物通商城首頁 > 試劑 > 分子生物學 > 報告系統/蛋白标記定位 > SNAP-Cell

          SNAP-Surface 啟動試劑盒

          SNAP-Surface Starter Kit

          品牌:NEB 貨号:E9120S 立即咨詢 QQ 咨詢留言

          暫無報價:

          選擇包裝:
          庫存貨期:
          服  務:
          • 基因有限公司 發貨并提供售後服務

          Shelf Life(month):24
          産地:USA
          存儲條件:-20
          蛋白質标記系統啟動試劑盒提供了标記 SNAP-tag融合蛋白的所有必需組份。能在活細胞、固定細胞及體外将紅色或綠色熒光基團共價連接到目标蛋白上。每種啟動試劑盒包括編碼SNAP-tag的質粒、兩種光穩定的非細胞通透性熒光底物SNAP-Surface 488(綠色)和SNAP-Surface 549(紅色),阻斷劑和對照,可在活細胞表面或溶液/體外對目标蛋白進行熒光示蹤。試劑盒中還提供一種陽性對照質粒,其編碼有亞細胞定位明确的标簽蛋白(如:膜蛋白、核蛋白等)。如果必要,試劑盒還會提供一個與目的标簽相互作用的、非熒光的陰性對照阻斷劑。The SNAP-Surface Starter Kit contains a mammalian expression plasmid (pSNAPf) encoding the SNAP-tag flanked by restriction sites for cloning a gene of interest, and two non-cell-permeable fluorescent SNAP-tag substrates. A positive control plasmid (pSNAPf-ADRβ2), encoding a SNAP-tagged protein (beta-2 adrenergic receptor) with a well-characterized cell surface localization, is also included. Lastly, a negative control “blocking agent” (SNAP-Surface Block) is included that interacts with the SNAP-tag, but is not fluorescent. There are two steps to using this system: subcloning and expression of the protein of interest as a SNAPf fusion, and labeling of the fusion with the SNAP-tag substrate of choice.

          The SNAP-tag® is a novel tool for the specific, covalent attachment of virtually any molecule to a protein of interest, providing simplicity and extraordinary versatility to the imaging of proteins in live and fixed cells, and to the study of proteins in vitro. The creation of a single gene construct yields a tagged fusion protein capable of forming a covalent linkage to a variety of functional groups, including fluorophores, biotin, or beads. This system provides a powerful and unique tool to study the role of proteins in a variety of highly dynamic processes, including protein trafficking, turnover and complex formation.

          The SNAP-tag is a 20 kDa mutant of the human DNA repair protein O6-alkylguanine- DNA alkyltransferase (hAGT) that reacts specifically and rapidly with benzylguanine (BG) and benzylchloropyrimidine (CP) derivatives, leading to covalent labeling of the SNAP-tag with a synthetic probe (Figure 1). The SNAP-tag has a number of features that make it ideal for a variety of protein labeling applications. The rate of the reaction of the SNAP-tag with these derivatives is largely independent of the nature of the synthetic probe attached to BG, permitting the labeling of SNAP fusion proteins with a wide variety of functional groups. Many of these SNAP-tag substrates are cell-impermeable, allowing live-cell imaging of protein expression and localization on the cell surface (Figure 2). The ability to turn on the signal at will, together with the availability of a cell-impermeable nonfluorescent blocking agent (SNAP-Surface® Block), allows time-resolved pulse-chase analysis of protein trafficking to the cell surface, as well as subsequent internalization. Finally, the availability of orthogonal protein labeling systems from NEB permits simultaneous labeling of multiple proteins in a single cell (CLIP-tag™, a SNAP-tag variant that reacts exclusively with O2-benzylcytosine substrates, and the ACP/MCP tags, small protein tags which can be enzymatically labeled on the cell surface with Coenzyme A derivatives).
          • 産品咨詢
          • 咨詢标題:[咨詢産品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
          • 咨詢内容:
          • 聯系方式: (可以是手機,座機[帶區号],Email,QQ等)
          • 驗證碼:
          • 常見問題(0條)
          常見問題
          • 産品評價( 0 條)
          産品評價
          好評度 98%
          • 不錯
          • 不錯123
          全部評價(1)
           隻看當前商品評價
          張三

          黃金會員

          不錯不錯

          生物通 版權所有

          未經書面授權,所有頁面内容、圖片和設計不得以任何形式進行複制

          Copyright © 2000-2019 eBiotrade.com, All Rights Reserved

          本欄目客服聯系電話:廣州 020-85524840轉1060

          本欄目廣告聯系電話:廣州 020-87511980

          主編信箱:

          粵公網安備 44010602000434号 粵ICP備09063491号

          http://2pva0a.dnshc4h.top|http://tgc0nyf3.dnshc4h.top|http://if9cy9kk.dnshc4h.top|http://3d95vx04.dnshc4h.top|http://pdrmx2r.dnshc4h.top