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          生物通商城首頁 > 試劑 > 分子生物學 > 電泳 > Western Blot發光檢測

          Phototope-HRP Western Blot Detection System,

          品牌:CST 貨号:7072S 立即咨詢 QQ 咨詢留言

          商城價:

          ¥2742.00目錄價:¥3656.00

          特價促銷:

          ¥2742.00
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          庫存貨期:
          服  務:
          • 基因生物技術國際貿易(上海)有限公司 發貨并提供售後服務

          Shelf Life(month):12
          産地:USA
          存儲條件:-20C
          50次反應。試劑盒含發光底物,HRP偶聯的抗小鼠二抗和生物素标記蛋白分子量标記,靈敏度高,發光持續時間長,可檢測亞皮克級的蛋白質。為标準的western blotting 應用中檢測化學發光的蛋白而設計。蛋白和生物素化的分子量标記(已提供)可以在SDS-PAGE中分離,再被轉移到膜上。與抗血清的一抗和辣根過氧化物酶(HRP) 标記的二抗孵育後,HRP标記的抗生物素抗體被結合,然後與LumiGLO ® 試劑反應,發出的光随後被捕獲到X膠片上。
          Designed for the chemiluminescent detection of proteins in standard Western blotting applications. Proteins and biotinylated molecular weight markers (provided) are separated by SDS-PAGE and transferred onto membrane. Following incubation with your primary anti-serum, horseradish peroxidase (HRP) linked secondary antibody and HRP-linked anti-biotin antibody are bound and then allowed to react with LumiGLO® reagent. The light emitted by destabilized LumiGLO® reagent is subsequently captured on X-ray film. 50 assays.
          20X LumiGLO® Reagent and 20X Peroxide #7003 25 ml each
          Anti-mouse IgG, HRP-linked Antibody #7076 250 µl Goat
          Biotinylated Protein Ladder Detection Pack #7727 500 µl
          LumiGLO chemiluminescent substrate is a luminol-based system designed for use with our Phototope-HRP detection assays utilizing peroxidase-labeled antibodies immobilized on membranes. In the presence of hydrogen peroxide, horseradish peroxidase (HRP) converts luminol to an excited intermediate dianion. This dianion emits light on return to its ground state. Light emission is maximal immediately after exposure of the substrate to HRP and continues for 0.5-1 hour. Light can be captured on X-ray film, typically by exposure for a few seconds. Maximum sensitivity can be obtained by longer exposure. *Avoid repeated exposure to skin
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          • 咨詢标題:[咨詢産品]CST / 7072S / Phototope-HRP Western Blot Detection System,
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