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          生物通商城首頁 > 試劑 > 細胞生物學 > 細胞染料/标記示蹤 > 細胞核

          Propidium Iodide (PI)/RNase Staining Solution

          品牌:CST 貨号:4087S 立即咨詢 QQ 咨詢留言

          商城價:

          ¥1206.00目錄價:¥1608.00

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          ¥1206.00
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          • 基因有限公司 發貨并提供售後服務

          Shelf Life(month):12
          産地:USA
          存儲條件:+4C
          PI是一種熒光染料,可以插在基底和染色的DNA和RNA之間。特異的DNA染色時通過采用核糖核酸酶(RNase)清除RNA。PI/Rnase通常在熒光顯微鏡下染色細胞核,在流式細胞分析細胞周期時确定DNA的含量。處于細胞周期的G2和M期的細胞包含的DNA是G0和G1期細胞的2倍。S期的DNA含量處于兩者之間。PI/Rnase染色試劑的激發和發射光最大分别在535nm和617nm(在光譜的桔紅到紅色的範圍)。此染色試劑可以應與抗體串聯,無約束力的幹擾,适于多重檢測中的DNA染色. 在熒光染色的最後一步,加入足夠的未稀釋的PI/Rnase染色試劑到浸沒細胞或樣本,室溫孵育15分鐘,分析前避光。100ml.
          Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.
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