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          生物通商城首頁 > 試劑 > 分子生物學 > 純化提取 > DNA片段純化 > 膠回收/離心柱

          NucleoSpin 膠/PCR産物純化試劑盒

          NucleoSpin Gel and PCR Clean-up

          品牌:MNG 貨号:740609.50 立即咨詢 QQ 咨詢留言

          商城價:

          ¥619.00目錄價:¥1015.00

          特價促銷:

          ¥619.00
          選擇包裝:
          庫存貨期:
          服  務:
          • 基因有限公司 發貨并提供售後服務

          Shelf Life(month):48
          産地:Germany
          存儲條件:常溫
          膠回收與PCR 産物回收純化二合為一,高效回收大于50bp的片段。回收率高,洗脫體積小(15uL),從而可獲得高濃度的DNA回收産物。适用于TAE,TBE等各種膠緩沖系統。10分鐘完成,省時省力。适用于PCR 産物純化;從凝膠中抽提DNA 樣本;濃縮反應産物以及去除鹽離子;可以純化濃縮單鍊DNA (需要NTC 緩沖液);可以去除樣本中SDS 雜質(需要NTB 緩沖液)。純化後的産物适用于:克隆、測序、PCR、限制酶内切等實驗
          矽膠膜技術 微型離心柱
          樣本 <400 μL PCR 反應産物 < 400 mg TAE/TBE agarose膠
          回收片段大小 50bp – 15 kbp
          回收效率 70 – 95 %
          A260/A280: 1.8 – 1.9
          洗脫體積: 15 – 50 μL
          制備時間 10 min / 6 preps
          載量 25μ g
          PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume
          • Two applications in one kit, one buffer with optimal performance for both applications
          • High recoveries for fragments down to 50 bp
          • Minimized elution volume: 15 μL – highly concentrated DNA
          • Binding buffer with pH indicator
          • Separate buffers for single stranded DNA or SDS containing samples available
          • Suitable for all gel buffer systems (e.g., TAE, TBE)
          • Also available for QIAcube® (please see "Ordering information" and contact Technical Service
          at techbio@mn-net.com for more information).

          Technology: Silica-membrane technology
          Format: Mini spin columns
          Sample material: < 400 µL PCR reaction mixture 、 < 400 mg TAE / TBE agarose gel
          Fragment size: 50 bp–approx. 20 kbp
          Typical recovery: 70–95%
          A260/A280: 1.8–1.9
          Elution volume 15–30 µL
          Preparation time 10 min/6 preps
          Binding capacity 25 µg

          Applications
          • Purification of PCR products
          • Extraction of DNA from agarose gels
          • NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
          - nucleotides, primers
          - enzymes
          - mineral oil
          - PCR additives (e.g., salts, betaine, DMSO)
          - detergents (e.g., Tween 20, Triton X-100)
          - dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)
          • Support protocol for DNA extraction from polyacrylamide gels
          • Support protocol for clean-up of single stranded DNA and support protocol for RNA extraction from
          agarose gels (Binding Buffer NTC required, not included in the kit, see “Ordering information”)
          • Support protocol for clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
          kit, see “Ordering information”)

          • Typical downstream applications: cloning, sequencing, PCR, restriction analysis
          NucleoSpin® Gel and PCR Clean-up Columns,Collection Tubes (2 mL), buffers
          50 preps for PCR clean-up and gel extraction - NucleoSpin Gel and PCR Clean-up Columns, Collection Tubes, buffers

          • 産品咨詢
          • 咨詢标題:[咨詢産品]MNG / 740609.50 / NucleoSpin Gel and PCR Clean-up
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