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          生物通商城首頁 > 試劑 > 細胞生物學 > 活體成像試劑 > 生物發光熒光素底物

          XenoLight D-Luciferin生物發光底物

          XenoLight D-Luciferin - K+ Salt Bioluminescen

          品牌:PERKINELMER 貨号:122799 立即咨詢 QQ 咨詢留言

          熒光素能夠通過血腦屏障,血胎盤屏障和血睾丸屏障,毒性低。

          商城價:

          ¥3300.00目錄價:¥4125.00

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          ¥3300.00
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          産地:USA
          存儲條件:常溫
          發光類型:近紅外熒光
          熒光素是一種低分子量有機化合物,由苯并噻唑基和噻唑羧酸基組成。熒光素是在螢火蟲和和其他有熒光素酶動物中被發現的,在ATP和酶熒光素的催化下發光。熒光素分子較小,免疫原性低/抗原性低。熒光素能夠通過血腦屏障,血胎盤屏障和血睾丸屏障,毒性低。D-Luciferin是生物體的細胞中發現的一種化學物質,在熒光素酶和ATP的催化作用下産生光。熒光素能夠穿透細胞膜,可以用于監控體内轉導及表達熒光素酶的細胞活動,因為熒光素酶的反應是ATP依賴,可檢測細胞活性。
          Luciferin is a low molecular weight organic compound that consists of a benzothiazole moiety attached to a thiazole carboxylic acid moiety. Luciferin is found in fireflies and other animals which, in the presence of ATP and the enzyme luciferase, becomes luminescent. The small size of luciferin also makes it a poor antigen and immune responses to luciferin are unlikely. Luciferin is able to pass the blood brain barrier, the blood placenta barrier and the blood testis barrier, toxicity appears low.How do you administer luciferin? Mice with lux-bearing bacteria do not need luciferin to glow. In the tumor models and transgenic models, luciferin is administered intraperitoneally (concomitant with anesthesia). How well does luciferin distribute? Luciferin distributes quickly and easily throughout the animal. How do the animals respond to the repeated administration of luciferin substrate? Luciferin does not affect the animals deleteriously (no evidence of toxicological or immunological effects). Do you need to administer luciferin substrate to the animals before imaging? In bacteria, the entire luciferase operon is stably integrated on the chromosome. This eliminates the need for exogenous luciferin substrate in the bacterial models. The tumor models and transgenic models rely on the exogenous administration of luciferin.
          http://www.perkinelmer.com/lab-solutions/resources/docs/SOP_LuciferinPrep_InVitroInVivo_BLI-Assays.pdf
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